^{10th Annual} Medical Microbiology Summit & Expo June 21-22, 2017 London, UK

Cellular and Molecular Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran\r\n

Due to the high prevalence of Toxoplasma gondii infection has been attracting a lot of attention to its diagnosis and treatment. The use of pure antigens although in some show high sensitivity and specificity, however the challenges such as cross-reactivity still remain as diagnostic difficulties. In this study, three surface antigens (SAG) of T. gondii were employed for designing of a multiepitope antigen. The antigen was then expressed using E. coli BL21 (DE3) cells and purified by affinity chromatography. ELISA analysis was used for evaluation of acute toxoplasmosis. The results showed a sensitivity of 72.6% and a specificity of 90.3% for recombinant ELISA.\r\n