Mmuoegbulam Oluchi Augusta
University of Calabar, Nigeria
Title: Phylogenetic classification and antibiotic resistance study of hydrocarbon and non-hydrocarbon utilizing proteobacteria isolated from Iko River Estuary, Nigeria
Biography
Biography: Mmuoegbulam Oluchi Augusta
Abstract
The phylum Proteobacteria is made up of many characterized polar flagellated, Gram-negative, rod-shaped, aerobic bacteria with the genus Stenotrophomonas and Xanthomonas being accommodated in the family Xanthomonadaceae while the genus Pseudomonas belong to the family pseudomonadaceae. The 16S rRNA sequences of the 12 hydrocarbon and non-hydrocarbon utilizing Proteobacteria in this study were determined and matched with available sequences in the ribosomal database project (RDP). Only one isolate was found to belong to the family Pseudomonadaceae and was of the genus Pseudomonas (Specie: P. aerugonosa DSM 50071 THE978271) while 11 were found to belong to the family Xanthomonadaceae and were of the genus Stenotrophomonas. Among the 11 Stenotrophomonas, 6 were identified to be Pseudomonas beteli (Stenotrophomonas maltophilia ATCC 19861 TAB021406), 3 as Pseudomonas hibiscicola and 2 as Stenotrophomonas maltophilia IAM 12423 TAB294553). The blast comparison also showed the closeness of the genus Stenotrophomonas to the genus Pseudomonas in their 16S rRNA sequence as seen in P. beteli which is known as S. maltophilia, ATCC 19861. P. beteli, P. hibiscicola and S. maltophilia-IAM 12423 were all species of the genus Stenotrophomonas. The antibiotic resistance study of all the proteobacteria using gentamicin, ciprofloxacin and clavulin showed that P. Aerugonosa DSM 50071 was resistant to clavulin with a minimum inhibitory concentration of 2000 µg/ml and a minimum bactericidal concentration of 2000 µg/ml. P. beteli ATCC 19861 and Pseudomonas hibiscicola ATCC 19867 had low level resistance to gentamicin while P. aeruginosa DSM 50071 and S. maltophilia IAM 12423/S. pavanii ICB 89 were sensitive.